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Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using MARCKS (Phospho-Ser162).
Immunofluorescence staining of methanol-fixed HeLa cells using MARCKS (Phospho-Ser162).
Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using MARCKS (Phospho-Ser162).

MARCKS (phospho Ser162) Antibody

CATALOG NUMBER: 79-486

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.
Predicted Molecular Weight:
80 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MARCKS
Additional Names:
MACS, 80K-L, PKCSL, PRKCSL, TSFP1, MACS, MARCS, Protein kinase C substrate
Protein Accession Number:
NP_002347.5
Protein Gi Number:
153070260
Ncbi Gene Id Number:
4082
Background:
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Background Reference 1:
Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102 (35): 12407-12412Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280 (3): 605-609Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359 (2): 151-159

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-486

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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