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Western blot analysis of MARS Antibody in HL-60 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MARS antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of MARS Antibody in HL-60 cell line lysates (35ug/lane)

MARS Antibody

CATALOG NUMBER: 63-237

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 872-899 amino acids from the C-terminal region of human MARS.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
101 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MARS
Additional Names:
Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase, MetRS, MARS
Protein Accession Number:
P56192
Protein Gi Number:
20178332
Ncbi Gene Id Number:
4141
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction:ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Background Reference 1:
Kaminska,M., Biochemistry 40 (47), 14309-14316 (2001)
Background Reference 2:
Kang,J., J. Biol. Chem. 275 (41), 31682-31688 (2000)
Background Reference 3:
Ko,Y.G., J. Cell Biol. 149 (3), 567-574 (2000)
Background Reference 4:
Quevillon,S., J. Mol. Biol. 285 (1), 183-195 (1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-237

List Size:
400 ul

List Price:

$495.00

Datasheet
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