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Western blot analysis of extracts of SH-SY5Y cells, using MARVELD2 antibody (23-758) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse stomach using MARVELD2 antibody (23-758) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using MARVELD2 antibody (23-758) (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using MARVELD2 antibody (23-758) (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MARVELD2 antibody (23-758) (40x lens).
Western blot analysis of extracts of SH-SY5Y cells, using MARVELD2 antibody (23-758) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

MARVELD2 Antibody

CATALOG NUMBER: 23-758

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MARVELD2 (NP_001033692.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
SH-SY5Y
Predicted Molecular Weight:
Observed: 64kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MARVELD2
Additional Names:
MARVEL domain-containing protein 2, Tricellulin, MARVELD2, TRIC
Ncbi Gene Id Number:
153562
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a membrane protein found at the tight junctions between epithelial cells. The encoded protein helps establish epithelial barriers such as those in the organ of Corti, where these barriers are required for normal hearing. Defects in this gene are a cause of deafness autosomal recessive type 49 (DFNB49). Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-758

List Size:
100 uL

List Price:

$436.00

Datasheet
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