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Western blot analysis of lysate from Hela cell line,using Maspin Antibody. was diluted at 1:1000 at each lane
Maspin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of Maspin Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysate from Hela cell line,using Maspin Antibody. was diluted at 1:1000 at each lane

Maspin Antibody

CATALOG NUMBER: 56-219

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Maspin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-123 amino acids from the Central region of human Maspin.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SERPINB5
Additional Names:
Serpin B5, Maspin, Peptidase inhibitor 5, PI-5, SERPINB5, PI5
Protein Accession Number:
P36952
Protein Gi Number:
229462757
Ncbi Gene Id Number:
5268
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SERPINB5 is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Background Reference 1:
Kim, J., et al. Tumour Biol. 31(5):471-476(2010)
Background Reference 2:
Juengel, E., et al. WorldUrol 28(4):465-471(2010)
Background Reference 3:
Besgen, P., et al. J. Immunol. 184(9):5392-5402(2010)
Background Reference 4:
Fung, C.L., et al. Histopathology 56(3):319-330(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-219

List Size:
400 ul

List Price:

$519.00

Datasheet
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