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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
MAT1A Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

MAT1A Antibody

CATALOG NUMBER: 56-593

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Bovine
Immunogen:
This MAT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-136 amino acids from the N-terminal region of human MAT1A.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAT1A
Additional Names:
S-adenosylmethionine synthase isoform type-1, AdoMet synthase 1, Methionine adenosyltransferase 1, MAT 1, Methionine adenosyltransferase I/III, MAT-I/III, MAT1A, AMS1, MATA1
Protein Accession Number:
Q00266
Protein Gi Number:
417297
Ncbi Gene Id Number:
4143
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene catalyzes a two-step reaction that involves thetransfer of the adenosyl moiety of ATP to methionine to formS-adenosylmethionine and tripolyphosphate, which is subsequentlycleaved to PPi and Pi. S-adenosylmethionine is the source of methylgroups for most biological methylations. The encoded protein isfound as a homotetramer (MAT I) or a homodimer (MAT III) whereas athird form, MAT II (gamma), is encoded by the MAT2A gene. Mutationsin this gene are associated with methionine adenosyltransferasedeficiency.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Wu, S.M., et al. Cell. Mol. Life Sci. 67(11):1831-1843(2010)
Background Reference 3:
Lai, C.Q., et al. Am. J. Clin. Nutr. 91(5):1377-1386(2010)
Background Reference 4:
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-593

List Size:
400 ul

List Price:

$495.00

Datasheet
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