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Western blot analysis of MATN4 in rat brain tissue lysate with MATN4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of MATN4 in mouse brain tissue with MATN4 antibody at 2.5 μg/mL.
Immunofluorescence of MATN4 in mouse brain tissue with MATN4 antibody at 20 μg/mL.
Western blot analysis of MATN4 in rat brain tissue lysate with MATN4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

MATN4 Antibody

CATALOG NUMBER: 5115

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MATN4 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human MATN4.
The immunogen is located within amino acids 110 - 160 of MATN4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MATN4 antibody can be used for detection of MATN4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
MATN4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MATN4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MATN4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MATN4
Additional Names:
MATN4 Antibody: Matrilin-4
Protein Accession Number:
NP_085080
Protein Gi Number:
13699834
Ncbi Gene Id Number:
8785
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MATN4 Antibody: Matrilin (MATNs) are a family of non-collagenous extracellular matrix proteins consisting of four known members that have been proposed to play key roles in the formation of both collagen-dependent and collagen-independent filamentous networks. Members of the matrilin family all share a structure made up of von Willebrand factor A domains, epidermal growth factor-like domains and a coiled coil alpha-helical module. MATN1 and MATN3 are expressed mainly in cartilage, while MATN2 and MATN4 occur in a wide variety of extracellular matrices such as embryonic kidney, lung, bone, cartilage and nervous system. The matrilin genes are strictly and differently regulated and their expression may serve as markers for cellular differentiation. MATN4 could serve as an odontoblast differentiation marker, e.g. in odontoblast stem cell research.
Background Reference 1:
Pei M, Luo J, and Chen Q. Enhancing and maintaining chondrogenesis of synovial fibroblasts by cartilage extracellular matrix protein matrilins. Osteoarthritis Cartilage2008; 16:1110-7.
Background Reference 2:
Frank S, Schulthess T, Landwehr R, et al. Characterization of the Matrilin Coiled-coil Domains Reveals Seven Novel Isoforms. J. Biol. Chem.2002; 277:19071-9.
Background Reference 3:
Deak F, Wagener R, Kiss I, et al. The matrilins: a novel family of oligomeric extracellular matrix proteins. Matrix Biol.1999; 18:55-64.
Background Reference 4:
Klatt AR, Nitche DP, Kobbe B, et al. Molecular structure, processing, and tissue distribution of matrilin. J. Biol. Chem.2001; 276:17267-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5115

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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