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Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Antibody is used in Western blot to detect MBD2 in A375 cell lysate (lane 1) and mouse brain tissue lysate (lane 2) lysate.
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

MBD2 Antibody

CATALOG NUMBER: 55-334

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-151 amino acids from the N-terminal region of human MBD2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MBD2
Additional Names:
Methyl-CpG-binding domain protein 2, Demethylase, DMTase, Methyl-CpG-binding protein MBD2, MBD2
Protein Accession Number:
Q9UBB5
Protein Gi Number:
50401198
Ncbi Gene Id Number:
8932
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Background Reference 1:
Zhu, Y., et al., Cancer 100(9):1853-1858 (2004).
Background Reference 2:
Ghoshal, K., et al., J. Biol. Chem. 279(8):6783-6793 (2004).
Background Reference 3:
Fujita, H., et al., Mol. Cell. Biol. 23(8):2645-2657 (2003).
Background Reference 4:
Patra, S.K., et al., Biochem. Biophys. Res. Commun. 302(4):759-766 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-334

List Size:
400 ul

List Price:

$495.00

Datasheet
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