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Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).

MBD4 Antibody

CATALOG NUMBER: 60-515

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the Central region of human MBD4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MBD4
Additional Names:
Methyl-CpG-binding domain protein 4, 322-, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, MBD4, MED1
Protein Accession Number:
O95243
Ncbi Gene Id Number:
8930
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DNA methylation is the major modification of eukaryoticgenomes and plays an essential role in mammalian development. Humanproteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family ofnuclear proteins related by the presence in each of a methyl-CpGbinding domain (MBD). Each of these proteins, with the exception ofMBD3, is capable of binding specifically to methylated DNA. MBD4may function to mediate the biological consequences of themethylation signal. In addition, MBD4 has protein sequencesimilarity to bacterial DNA repair enzymes and thus may have somefunction in DNA repair. Further, MBD4 gene mutations are detectedin tumors with primary microsatellite-instability (MSI), a form ofgenomic instability associated with defective DNA mismatch repair,and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.
Background Reference 1:
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Background Reference 2:
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
Background Reference 3:
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
Background Reference 4:
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-515

List Size:
400 ul

List Price:

$495.00

Datasheet
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