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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis of MBL2 using rabbit polyclonal MBL2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MBL2 gene.
Confocal immunofluorescent analysis of MBL2 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
MBL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in A549 cell line lysates (35ug/lane).

MBL2 Antibody

CATALOG NUMBER: 57-234

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MBL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-202 amino acids from the C-terminal region of human MBL2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MBL2
Additional Names:
Mannose-binding protein C, MBP-C, Collectin-1, MBP1, Mannan-binding protein, Mannose-binding lectin, MBL2, COLEC1, MBL
Protein Accession Number:
P11226
Protein Gi Number:
126676
Ncbi Gene Id Number:
4153
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the soluble mannose-binding lectin ormannose-binding protein found in serum. The protein encoded belongsto the collectin family and is an important element in the innateimmune system. The protein recognizes mannose andN-acetylglucosamine on many microorganisms, and is capable ofactivating the classical complement pathway. Deficiencies of thisgene have been associated with susceptibility to autoimmune andinfectious diseases.
Background Reference 1:
Auriti, C., et al. Hum. Immunol. 71(11):1084-1088(2010)
Background Reference 2:
Fraser, D.A., et al. J. Immunol. 185(7):3932-3939(2010)
Background Reference 3:
de Wit, E., et al. Mamm. Genome (2010) In press :
Background Reference 4:
Filho, R.M., et al. Viral Immunol. 23(4):449-453(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-234

List Size:
400 ul

List Price:

$469.00

Out of stock

Datasheet
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