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Western Blot at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A549 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

MBOAT7 Antibody

CATALOG NUMBER: 60-664

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Bovine
Immunogen:
This MBOAT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human MBOAT7.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MBOAT7
Additional Names:
Lysophospholipid acyltransferase 7, LPLAT 7, 231-, 1-acylglycerophosphatidylinositol O-acyltransferase, 231n4, Bladder and breast carcinoma-overexpressed gene 1 protein, Leukocyte receptor cluster member 4, Lysophosphatidylinositol acyltransferase, LPIAT, Lyso-PI acyltransferase, Membrane-bound O-acyltransferase domain-containing protein 7, O-acyltransferase domain-containing protein 7, h-mboa-7, MBOAT7, BB1, LENG4, OACT7
Protein Accession Number:
Q96N66
Protein Gi Number:
167008974
Ncbi Gene Id Number:
79143
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the membrane-boundO-acyltransferases family of integral membrane proteins that haveacyltransferase activity. The encoded protein is alysophosphatidylinositol acyltransferase that has specificity forarachidonoyl-CoA as an acyl donor. This protein is involved in thereacylation of phospholipids as part of the phospholipid remodelingpathway known as the Land cycle. Alternative splicing results inmultiple transcript variants.
Background Reference 1:
Gijon, M.A., et al. J. Biol. Chem. 283(44):30235-30245(2008)
Background Reference 2:
Lee, H.C., et al. Mol. Biol. Cell 19(3):1174-1184(2008)
Background Reference 3:
Wende, H., et al. Immunogenetics 51 (8-9), 703-713 (2000) :
Background Reference 4:
Fukunaga-Johnson, N., et al. Anticancer Res. 16 (3A), 1085-1090 (1996) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-664

List Size:
400 ul

List Price:

$495.00

Datasheet
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