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Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of MC4R in human brain tissue with MC4R antibody at 2.5 μg/mL.
Immunofluorescence of MC4R in Human Brain cells with MC4R antibody at 20 μg/mL.
Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

MC4R Antibody

CATALOG NUMBER: 5199

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (79%)
Immunogen:
MC4R antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human MC4R.
The immunogen is located within the first 50 amino acids of MC4R.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MC4R antibody can be used for detection of MC4R by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
MC4R Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MC4R Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MC4R antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MC4R
Additional Names:
MC4R Antibody: Melanocortin receptor 4, MC4-R
Protein Accession Number:
NP_005903
Protein Gi Number:
119508433
Ncbi Gene Id Number:
4160
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MC4R Antibody: The melanocortin-4 receptor (MC4R) is a member of the superfamily of seven transmembrane G-protein coupled receptors that are involved in multiple signal transduction pathways including the cAMP and MAPK signaling pathways. It is thought that the melanocortin system modulates energy expenditure and insulin sensitivity; activation of the MC4R results in the inhibition of c-Jun N-terminal kinase (JNK) activity and promotes insulin signaling. MC4R-null mice display maturity onset obesity characterized by hyperphagia, increased adiposity, hyperinsulinaemia and hyperleptinaemia, suggesting that like other obesity-linked genes such as FTO, PTER, and NPC1, MC4R is a potential candidate target for the treatment of obesity.
Background Reference 1:
Gantz I, Miwa H, Konda Y, et al. Molecular cloning, expression, and gene localization of a fourth melanocortin receptor. J. Biol. Chem.1993; 268:15174-9.
Background Reference 2:
Vongs A, Lynn NM, and Rosenblum CI. Activation of MAP kinase by MC4-R through PI3 kinase. Regul. Pept.2004; 120:113-8.
Background Reference 3:
Cone H. Anatomy and regulation of the central melanocortin system. Nat. Neurosci.2005; 8:571-8.
Background Reference 4:
Chai B, Li J-Y, Zhang W, et al. Melanocartin-4 receptor activation inhibits c-Jun N-terminal kinase activity and promotes insulin signaling. Peptides2009; 30:1098-10

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5199

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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