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Western blot analysis of MCAM Antibody in A2058 cell line lysates (35ug/lane)
MCAM Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of MCAM Antibody in A2058 cell line lysates (35ug/lane)

MCAM Antibody

CATALOG NUMBER: 61-494

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 532-558 amino acids from the C-terminal region of human MCAM.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
72 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCAM
Additional Names:
Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18
Protein Accession Number:
P43121
Protein Gi Number:
85681878
Ncbi Gene Id Number:
4162
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. It could be an adhesion molecule active in neural crest cells during embryonic development. It acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
Background Reference 1:
Fritzsche,F.R., Pathology 40 (5), 457-464 (2008)
Background Reference 2:
Malyszko,J., Clin. Appl. Thromb. Hemost. 14 (3), 338-345 (2008)
Background Reference 3:
Guezguez,B., J. Immunol. 179 (10), 6673-6685 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-494

List Size:
400 ul

List Price:

$495.00

Datasheet
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