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Western blot analysis in K562 cell line lysates (35ug/lane).
MCF2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

MCF2 Antibody

CATALOG NUMBER: 56-509

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MCF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 804-833 amino acids from the C-terminal region of human MCF2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
108 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCF2
Additional Names:
Proto-oncogene DBL, Proto-oncogene MCF-2, MCF2-transforming protein, DBL-transforming protein, MCF2, DBL
Protein Accession Number:
P10911
Protein Gi Number:
92087039
Ncbi Gene Id Number:
4168
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The oncogenic protein encoded by this gene is a guaninenucleotide exchange factor (GEF) that exerts control over somemembers of the Rho family of small GTPases. Several transcriptvariants encoding different isoforms have been found for this gene.These isoforms exhibit different expression patterns and varyinglevels of GEF activity.
Background Reference 1:
Piton, A., et al. Mol. Psychiatry (2010) In press :
Background Reference 2:
Murakami, M., et al. Int. J. Cancer 123(3):500-510(2008)
Background Reference 3:
Murakami, M., et al. Cancer Biol. Ther. 7(5):677-688(2008)
Background Reference 4:
Rojas, R.J., et al. J. Biol. Chem. 282(40):29201-29210(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-509

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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