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43-320 (1ug/ml) staining of Jurkat (A) and Human Thymus (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-320 (1ug/ml) staining of Jurkat (A) and Human Thymus (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MCF2L Antibody

CATALOG NUMBER: 43-320

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QLDNQATVQR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in lysates of cell line Jurkat and of Human Thymus (calculated MW of 131kDa according to NP_001106203.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001106203.1; NP_079255.3).
Predicted Molecular Weight:
Approx 150kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MCF2L
Additional Names:
MCF2L, MCF.2 cell line derived transforming sequence-like, ARHGEF14, DBS, FLJ12122, KIAA0362, OST, DBL's big sister, MCF2 transforming sequence-like protein, guanine nucleotide exchange factor DBS
Protein Accession Number:
NP_001106203.1,NP_079255.3
Protein Gi Number:
148887400
Ncbi Gene Id Number:
23263
Background Reference 1:
Takefuji M, Asano H, Mori K, Amano M, Kato K, Watanabe T, Morita Y, Katsumi A, Itoh T, Takenawa T, Hirashiki A, Izawa H, Nagata K, Hirayama H, Takatsu F, Naoe T, Yokota M, Kaibuchi K, Mutation of ARHGAP9 in patients with coronary spastic angina.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-320

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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