Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunocytochemistry of MCG10 in HeLa cells with MCG10 antibody at 1 μg/mL.
Immunofluorescence of MCG10 in Hela cells with MCG10 antibody at 20 μg/mL.
Western blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Green Product
Trial Size

MCG10 Antibody

CATALOG NUMBER: 2479

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%), Bovine: (100%)
Immunogen:
MCG10 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10.
The immunogen is located within the last 50 amino acids of MCG10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
MCG10 antibody can be used for detection of MCG10 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
MCG10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
MCG10 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
MCG10 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCBP4
Additional Names:
MCG10 Antibody: CBP, LIP4, MCG10Alpha-CP4
Protein Accession Number:
EAW65166
Protein Gi Number:
119585570
Ncbi Gene Id Number:
57060
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MCG10 Antibody: MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.
Background Reference 1:
Makeyev AV and Liebhaber SA. The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms. RNA2002; 8:265-78.
Background Reference 2:
Zhu J and Chen X. MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is capable of inducing apoptosis and cell cycle arrest in G2-M. Mol. Cell Biol.2000; 20:5602-5618.
Background Reference 3:
Pio R, Zudaire I, Pino I, et al. a-CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant aCP-4a, is underexpressed in lung cancer. Cancer Res.2004; 64:4171-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2479

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369