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Western blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunocytochemistry of MCG10 in HeLa cells with MCG10 antibody at 1 μg/mL.
Immunofluorescence of MCG10 in Hela cells with MCG10 antibody at 20 μg/mL.
Western blot analysis of MCG10 in HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

MCG10 Antibody

CATALOG NUMBER: 2479

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%), Bovine: (100%)
Immunogen:
MCG10 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MCG10.
The immunogen is located within the last 50 amino acids of MCG10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
MCG10 antibody can be used for detection of MCG10 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
MCG10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MCG10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
MCG10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PCBP4
Additional Names:
MCG10 Antibody: CBP, LIP4, MCG10Alpha-CP4
Protein Accession Number:
EAW65166
Protein Gi Number:
119585570
Ncbi Gene Id Number:
57060
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MCG10 Antibody: MCG10 is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. MCG10 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of MCG10 has been observed in many forms of lung cancer, leading to speculation that MCG10 can act as a tumor suppressor. Multiple alternatively spliced isoforms of MCG10 have been identified.
Background Reference 1:
Makeyev AV and Liebhaber SA. The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms. RNA2002; 8:265-78.
Background Reference 2:
Zhu J and Chen X. MCG10, a novel p53 target gene that encodes a KH domain RNA-binding protein, is capable of inducing apoptosis and cell cycle arrest in G2-M. Mol. Cell Biol.2000; 20:5602-5618.
Background Reference 3:
Pio R, Zudaire I, Pino I, et al. a-CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant aCP-4a, is underexpressed in lung cancer. Cancer Res.2004; 64:4171-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2479

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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