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Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
MCHR1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CEM,K562 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

MCHR1 Antibody

CATALOG NUMBER: 55-342

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MCHR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-422 amino acids from the C-terminal region of human MCHR1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCHR1
Additional Names:
Melanin-concentrating hormone receptor 1, MCH receptor 1, MCH-R1, MCHR-1, G-protein coupled receptor 24, MCH-1R, MCH1R, MCHR, SLC-1, Somatostatin receptor-like protein, MCHR1, GPR24, SLC1
Protein Accession Number:
Q99705
Protein Gi Number:
33860175
Ncbi Gene Id Number:
2847
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene, a member of the Gprotein-coupled receptor family 1, is an integral plasma membraneprotein which binds melanin-concentrating hormone. The encodedprotein can inhibit cAMP accumulation and stimulate intracellularcalcium flux, and is probably involved in the neuronal regulationof food consumption. Although structurally similar to somatostatinreceptors, this protein does not seem to bind somatostatin.
Background Reference 1:
Miyamoto-Matsubara, M., et al. Ann. N. Y. Acad. Sci. 1200, 112-119 (2010) :
Background Reference 2:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Background Reference 3:
Miller, C.L., et al. Schizophr. Res. 113 (2-3), 259-267 (2009) :
Background Reference 4:
Gavalas, N.G., et al. Exp. Dermatol. 18(5):454-463(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-342

List Size:
400 ul

List Price:

$495.00

Datasheet
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