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Western blot analysis of Mcl-1 in Raji cell lysates with Mcl-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunocytochemistry of Mcl-1 in Raji cells with Mcl-1 antibody at 10 μg/mL.
Western blot analysis of Mcl-1 in Raji cell lysates with Mcl-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

Mcl-1 Antibody

CATALOG NUMBER: 3481

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (81%), Rat: (79%)
Immunogen:
Mcl-1 antibody was raised against a peptide corresponding to 16 amino acids near the center of human Mcl-1.
The immunogen is located within amino acids 110 - 160 of Mcl-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
Mcl-1 antibody can be used for detection of isoforms Mcl-1L and Mcl-1S by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
Detects isoforms Mcl-1L and Mcl-1S
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Positive Control 2:
Cat. No. 17-007 - Raji Cell Slide
Purification:
Mcl-1 Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Mcl-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Mcl-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCL1
Additional Names:
Mcl-1 Antibody: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3
Protein Accession Number:
NP_068779
Protein Gi Number:
11386165
Ncbi Gene Id Number:
4170
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mcl-1 Antibody: Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival. While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. Mcl-1 exists as at least three distinct isoforms designated Mcl-1L, Mcl-1S and Mcl-1ES. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.
Background Reference 1:
Edwards SW, Derouet M, Howse M, et al. Regulation of neutrophil apoptosis by Mcl-1. Biochem Soc Trans. 2004; 32:489-92.
Background Reference 2:
Cuconati A, Mukherjee C, Perez D, et al. DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells. Genes and Dev. 2003; 17:2922-32.
Background Reference 3:
Opferman JT, Letai A, Beard C, et al. Development and maintenance of B and T lymphocytes require antiapoptotic MCL-1. Nature 2003; 426:671-6.
Background Reference 4:
Kozopas KM, Yang T, Buchan HL, et al. MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. Proc. Natl. Acad. Sci. USA 1993; 90:3516-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3481

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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