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Western blot analysis using MCL - 1 monoclonal antibody against BCBL - 1 cell lysate.
Immunohistochemical analysis of paraffin - embedded human lymphnode showing cytoplasmic localization using MCL - 1 antibody with DAB staining.
Western blot analysis using MCL - 1 monoclonal antibody against BCBL - 1 cell lysate.

MCL1 Antibody [8C6D4B1]

CATALOG NUMBER: 32-191

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant protein MCL-1expressed in E. Coli strain M15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:Dilution 1:200 - 1:1,000
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein G affinity chromatography.

Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
MCL-1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCL1
Additional Names:
TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT
Protein Accession Number:
NP_001184249.1
Protein Gi Number:
309747067
Ncbi Gene Id Number:
4170
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.
Background Reference 1:
Ota, N. et al. J. Hum. Genet. 2000. 46: 254-269.
Background Reference 2:
Schwertfeger KL, Ryder JW, Anderson SM J Mammary Gland Biol Neoplasia 2000, 3 : 236-251.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-191

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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