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Western blot analysis of extracts of various cell lines, using MCL1 antibody (13-878).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human pancreas using MCL1 antibody (13-878) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using MCL1 antibody (13-878) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using MCL1 antibody (13-878) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HepG2 cells using MCL1 antibody (13-878).
Immunofluorescence analysis of HeLa cells using MCL1 antibody (13-878).
Immunofluorescence analysis of BT-20 cells using MCL1 antibody (13-878).
Western blot analysis of extracts of various cell lines, using MCL1 antibody (13-878).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MCL1 Antibody [E8E5]

CATALOG NUMBER: 13-878

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human MCL1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
Raji
Positive Control 2:
Mouse heart
Predicted Molecular Weight:
Observed: 37kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MCL1
Additional Names:
Mcl-1 Antibody: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl2-L-3
Ncbi Gene Id Number:
4170
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-878

List Size:
50 uL

List Price:

$350.00

Datasheet
Shipping Info

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