MCM2 Antibody

CATALOG NUMBER: 28-535

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MCM2 antibody can be used for detection of MCM2 by ELISA at 1:312500. MCM2 antibody can be used for detection of MCM2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
99 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MCM2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MCM2
Additional Names:
MCM2, BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN
Protein Accession Number:
NP_004517
Protein Gi Number:
33356547
Ncbi Gene Id Number:
4171
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Background Reference 1:
Yu, Z., et al., (2004) J. Mol. Biol. 340 (5), 1197-1206.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-535

List Size:
100 ul

List Price:

$473.00

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