Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of MCM2 Antibody in HL-60 cell line lysates (35ug/lane)
MCM2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 ug per lane.

MCM2 Antibody

CATALOG NUMBER: 63-916

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus
Immunogen:
This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649-677 amino acids from the C-terminal region of human MCM2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
102 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCM2
Additional Names:
DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, BM28, CCNL1, CDCL1, KIAA0030
Protein Accession Number:
P49736
Protein Gi Number:
41019490
Ncbi Gene Id Number:
4171
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Background Reference 1:
Saade, E., et al. Proteomics 9(21):4934-4943(2009)
Background Reference 2:
Xu, X., et al. EMBO J. 28(19):3005-3014(2009)
Background Reference 3:
Liaw, K., et al. J. Cutan. Pathol. 36(10):1121-1122(2009)
Background Reference 4:
Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-916

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369