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Western blot analysis in CEM cell line lysates (35ug/lane).
MCM4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

MCM4 Antibody

CATALOG NUMBER: 57-795

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Mouse
Immunogen:
This MCM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the C-terminal region of human MCM4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MCM4
Additional Names:
DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21, MCM4, CDC21
Protein Accession Number:
P33991
Protein Gi Number:
68571766
Ncbi Gene Id Number:
4173
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is one of the highlyconserved mini-chromosome maintenance proteins (MCM) that areessential for the initiation of eukaryotic genome replication. Thehexameric protein complex formed by MCM proteins is a key componentof the pre-replication complex (pre_RC) and may be involved in theformation of replication forks and in the recruitment of other DNAreplication related proteins. The MCM complex consisting of thisprotein and MCM2, 6 and 7 proteins possesses DNA helicase activity,and may act as a DNA unwinding enzyme. The phosphorylation of thisprotein by CDC2 kinase reduces the DNA helicase activity andchromatin binding of the MCM complex. This gene is mapped to aregion on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, aDNA-activated protein kinase involved in the repair of DNAdouble-strand breaks. Alternatively spliced transcript variantsencoding the same protein have been reported.
Background Reference 1:
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Background Reference 2:
Qian, Z., et al. PLoS Pathog.(3), E1000814 (2010) :
Background Reference 3:
Ladstein, R.G., et al. BMC Cancer 10, 140 (2010) :
Background Reference 4:
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-795

List Size:
400 ul

List Price:

$495.00

Datasheet
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