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Western blot analysis of extracts of various cell lines, using MCM5 antibody (19-729) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using MCM5 antibody (19-729). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MCM5 antibody (19-729) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

MCM5 Antibody

CATALOG NUMBER: 19-729

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:1000 - 1:3000
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
Jurkat
Positive Control 3:
SW620
Positive Control 4:
Mouse spleen
Positive Control 5:
Rat thymus
Predicted Molecular Weight:
Observed: 100kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MCM5
Additional Names:
MCM5, CDC46, P1-CDC46
Ncbi Gene Id Number:
4174
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-729

List Size:
100 uL

List Price:

$436.00

Datasheet
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