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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

MCM5 Antibody

CATALOG NUMBER: 28-541

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MCM5 antibody can be used for detection of MCM5 by ELISA at 1:62500. MCM5 antibody can be used for detection of MCM5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
82 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MCM5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MCM5
Additional Names:
MCM5, CDC46, P1-CDC46
Protein Accession Number:
NP_006730
Protein Gi Number:
23510448
Ncbi Gene Id Number:
4174
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Background Reference 1:
Ohtani, K., et al., (1999) Oncogene 18 (14), 2299-2309.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-541

List Size:
100 ul

List Price:

$473.00

Datasheet
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