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Antibody used in IHC on Human Lung.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in IHC on Human Lung.

MCM6 Antibody

CATALOG NUMBER: 28-538

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
MCM6 antibody can be used for detection of MCM6 by ELISA at 1:62500. MCM6 antibody can be used for detection of MCM6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
90 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MCM6 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MCM6
Additional Names:
MCM6, Mis5, P105MCM, MCG40308
Protein Accession Number:
NP_005906
Protein Gi Number:
7427519
Ncbi Gene Id Number:
4175
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by the MCM6 gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication.
Background Reference 1:
Yabuta, N., et al., (2003) Genes Cells 8 (5), 413-421.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-538

List Size:
100 ul

List Price:

$473.00

Datasheet
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