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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human HepG2 at 1.25 ug/ml.

MCM7 Antibody

CATALOG NUMBER: 28-544

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MCM7 antibody can be used for detection of MCM7 by ELISA at 1:12500. MCM7 antibody can be used for detection of MCM7 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
42 kDa, 81 kDa, 61 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MCM7 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MCM7
Additional Names:
MCM7, MCM2, CDC47, P85MCM, P1CDC47, PNAS146, P1.1-MCM3
Protein Accession Number:
P33993-2
Protein Gi Number:
20981696
Ncbi Gene Id Number:
4176
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.
Background Reference 1:
Wang, Y., et al., (2000) FEBS Lett. 484 (1), 17-21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-544

List Size:
100 ul

List Price:

$473.00

Datasheet
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