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Western blot testing of human 1) HeLa, 2) Raji and 3) HepG2 cell lysate with MCM7 antibody (clone MCM7/1466). Expected molecular weight: 80-90 kDa.
IHC testing of FFPE human tonsil with MCM7 antibody (clone MCM7/1466). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free MCM7 Antibody (clone MCM7/1466). Confirmation of Integrity and Purity of the Antibody.
Western blot testing of human 1) HeLa, 2) Raji and 3) HepG2 cell lysate with MCM7 antibody (clone MCM7/1466). Expected molecular weight: 80-90 kDa.

MCM7 Antibody [MCM7/1466]

CATALOG NUMBER: 33-725

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 195-319 were used as the immunogen for the MCM7 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
IF: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
MCM7
Additional Names:
DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2
Ncbi Gene Id Number:
4176
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-725

List Size:
100 ug

List Price:

$626.00

Datasheet
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