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Western blot analysis using MCP - 1 monoclonal antibody against truncated MCP - 1 recombinant protein.
Western blot analysis using MCP - 1 monoclonal antibody against truncated MCP - 1 recombinant protein.

MCP-1 Antibody [1A7B8]

CATALOG NUMBER: 32-230

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human MCP-1 expressed in E.Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1,000
ELISA:1,000 - 2,000.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1.kappa
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
to MCP-1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CCL2
Additional Names:
MCP1, SCYA2, HC11, MCAF, MCP1, MCP-1, GDCF-2, SMC-CF, HSMCR30
Protein Accession Number:
P13500
Protein Gi Number:
126842
Ncbi Gene Id Number:
6347
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Monocyte chemoattractant protein-1 (MCP-1) is a member of the chemokine-beta family of cytokines. The protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. It may play a inprotant role in the initiation and/or progression of pulmonary hypertension (PH). Blockade of a systemic MCP-1 signal pathway in vivo may prevent PH.
Background Reference 1:
Yoshimura T. et al. 1989. FEBS Lett. 244:487-493.
Background Reference 2:
Yoshimura T. et al. 1991. Adv. Exp. Med. Biol. 305:47-56.
Background Reference 3:
Rollins B.J. et al. 1991. Genomics. 10:489-492.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-230

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

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