Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat MCP-1(MCAF) (XP-5219Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).
To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat MCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1(MCAF) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat MCP-1(MCAF) (XP-5219Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).

MCP-1/MCAF Antibody

CATALOG NUMBER: XP-5219

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1/MCAF (Macrophage/Monocyte chemotactic protein-1).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat MCP-1/MCAF (100 ng/mL), a concentration of 2.0 - 4.0 μg/mL of this antibody is required.
ELISA:
To detect Rat MCP-1/MCAF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MCP-1/MCAF.
Sandwich:
To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MCP-1(MCAF) (XP-5219Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1(MCAF).
Western Blot:
To detect Rat MCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1/MCAF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-Rat MCP-1/MCAF specific antibody was purified by affinity chromatography employing immobilized Rat MCP-1/MCAF matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Ccl2
Additional Names:
MCP-1, Scya2, Sigje, Je, Mcp1, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, MCP-1
Protein Accession Number:
P14844
Protein Gi Number:
126846
Ncbi Gene Id Number:
24770
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5219

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369