MCP-3 Antibody (biotin)

CATALOG NUMBER: XP-5223Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Biotin
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mMCP-3 (murine MCP-3).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect mMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine MCP-3 (XP-5223) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.
Western Blot:
To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-mMCP-3 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
MCP-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Ccl7
Additional Names:
fic, marc, mcp3, MCP-3, Scya7, Fic, Mcp3, C-C motif chemokine 7, Monocyte chemoattractant protein 3
Protein Accession Number:
Q03366
Protein Gi Number:
417192
Ncbi Gene Id Number:
20306
User Note:
Centrifuge vial prior to opening.
Background:
This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5223Bt

List Size:
0.05 mg

List Price:

$408.00

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