MCP-3 Antibody [PEPY042_hMCP3]

Application Note:

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-3 (100ng/mL), a concentration of 1 - 3 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect recombinant human MCP-3 when used with our biotinylated antigen affinity purified anti-human MCP-3 (XP-5221Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.