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Western blot analysis of extracts of mouse seminoma, using MCT1 antibody (18-906).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of various cells, using MCT1 antibody (18-906) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat pancreas using MCT1 antibody (18-906) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MCT1 antibody (18-906) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MCT1 antibody (18-906) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using MCT1 antibody (18-906) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse seminoma, using MCT1 antibody (18-906).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MCT1 Antibody

CATALOG NUMBER: 18-906

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human MCT1
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:1000 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse seminoma
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC16A1
Additional Names:
SLC16A1, FLJ36745, MCT, MCT1, MGC44475, HHF7
Ncbi Gene Id Number:
6566
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-906

List Size:
100 uL

List Price:

$436.00

Datasheet
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