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Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.
Immunofluorescence of MD2 in human spleen tissue with MD2 antibody at 20 μg/mL.
Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.
Trial Size

MD-2 Antibody [9F1B1]

CATALOG NUMBER: PM-4845

Clonality:
Monoclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 95-108 - MD-2 Recombinant Protein
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
MD-2 Monoclonal Antibody is Protein A purified.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MD-2 monoclonal antibody can be stored at -20°C, stable for one year.
Ncbi Official Symbol:
LY96
Additional Names:
MD-2 Antibody [9F1B1] : MD2, MD-2, ly-96, ESOP-1
Protein Accession Number:
NP_056179
Protein Gi Number:
223555998
Ncbi Gene Id Number:
23643
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MD-2 Monoclonal Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Background Reference 1:
O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
Background Reference 2:
Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
Background Reference 3:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 4:
Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-4845

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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