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Western blot analysis of extracts of various cell lines, using MDC1 antibody (23-531) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody (23-531) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using MDC1 antibody (23-531) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MDC1 antibody (23-531) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 5s.

MDC1 Antibody

CATALOG NUMBER: 23-531

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
LO2
Predicted Molecular Weight:
Observed: 270kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MDC1
Additional Names:
IER3, NFBD1, KIAA0170, DKFZp781A0122
Ncbi Gene Id Number:
9656
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-531

List Size:
100 uL

List Price:

$436.00

Datasheet
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