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42-165 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-165 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

MDFI Antibody

CATALOG NUMBER: 42-165

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SKSTTSQIPLQAQED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 70kDa band in lysates of cell line HepG2 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Lung (Bronchus). Recommended concentration: 5ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MDFI
Additional Names:
MDFI, MyoD family inhibitor, I-MF, I-mfa, OTTHUMP00000016375, OTTHUMP00000196414, inhibitor of MyoD family a
Protein Accession Number:
NP_005577.1
Protein Gi Number:
5031905
Ncbi Gene Id Number:
4188
Background Reference 1:
Ma R, Rundle D, Jacks J, Koch M, Downs T, Tsiokas L, Inhibitor of myogenic family, a novel suppressor of store-operated currents through an interaction with TRPC1. The Journal of biological chemistry 2003 Dec 278 (52): 52763-72.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-165

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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