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Western blot analysis of MDFIC Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human spleen reacted with MDFIC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
MDFIC Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MDFIC Antibody in Hela cell line lysates (35ug/lane)

MDFIC Antibody

CATALOG NUMBER: 63-605

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MDFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-99 amino acids from the N-terminal region of human MDFIC.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Names:
MyoD family inhibitor domain-containing protein, I-mfa domain-containing protein, hIC, MDFIC (HGNC:28870)
Protein Accession Number:
Q9P1T7
Protein Gi Number:
257051035
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MDFIC is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus.
Background Reference 1:
Thebault,S. et.al., Curr. Protein Pept. Sci.(2), 155-167 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-605

List Size:
400 ul

List Price:

$495.00

Datasheet
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