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Antibody used in WB on Human Jurkat 2.5 ug/ml.
Antibody used in WB on Human Jurkat 2.5 ug/ml.

MDH1 Antibody

CATALOG NUMBER: 30-390

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Arabidopsis, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Arabidopsis, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MDH1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MDH1 antibody can be used for detection of MDH1 by ELISA at 1:62500. MDH1 antibody can be used for detection of MDH1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
36 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MDH1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MDH1
Additional Names:
MDH1, MDH-s, MDHA, MGC:1375, MOR2, HEL-S-32
Protein Accession Number:
NP_005908
Protein Gi Number:
5174539
Ncbi Gene Id Number:
4190
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Hillier, L.W., (2005) Nature 434 (7034), 724-731.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-390

List Size:
100 ul

List Price:

$473.00

Datasheet
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