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Western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).

MDH1 Antibody

CATALOG NUMBER: 61-590

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Pig, Rat
Immunogen:
This MDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-314 amino acids from the C-terminal region of human MDH1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MDH1
Additional Names:
Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase, Diiodophenylpyruvate reductase, MDH1, MDHA
Protein Accession Number:
P40925
Protein Gi Number:
1708967
Ncbi Gene Id Number:
4190
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Background Reference 1:
Lee,S.M., et.al., Cell Death Differ. 16 (5), 738-748 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-590

List Size:
400 ul

List Price:

$495.00

Datasheet
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