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Western blot analysis in A549 cell line lysates (35ug/lane).
MDM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in A549 cell line lysates (35ug/lane).

MDM1 Antibody

CATALOG NUMBER: 56-361

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MDM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-684 amino acids from the C-terminal region of human MDM1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
81 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MDM1
Additional Names:
Nuclear protein MDM1, MDM1
Protein Accession Number:
Q8TC05
Protein Gi Number:
156632525
Ncbi Gene Id Number:
56890
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a nuclear protein similar to the mousedouble minute 1 protein. The mouse gene is located in double minute(DM) chromatin particles and is amplified in the mouse transformed3T3 cell line, and the protein is able to bind to p53. In mouseseveral transcripts have been described for this gene which resultfrom alternative polyadenylation, splicing and exon usage.
Background Reference 1:
Chang, B., et al. Hum. Mol. Genet. 17(24):3929-3941(2008)
Background Reference 2:
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Background Reference 3:
Snyder, L.C., et al. J. Biol. Chem. 263(32):17150-17158(1988)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-361

List Size:
400 ul

List Price:

$495.00

Datasheet
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