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Western blot analysis of lysed extracts from 293 and HeLa cells using MDM2 Antibody.
Western blot analysis of lysed extracts from 293 and HeLa cells using MDM2 Antibody.

MDM2 Antibody

CATALOG NUMBER: 79-653

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
MDM2 antibody was raised against a peptide sequence around aa.18~22 (Q-I-P-A-S) derived from Human MDM2. .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous levels of total MDM2 protein.
Predicted Molecular Weight:
90 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MDM2
Additional Names:
HDMX, hdm2, ACTFS, CCN5, CT58, CTGFL, UNQ228/PRO261, HDMX
Protein Accession Number:
NP_002383.2
Protein Gi Number:
89993689
Ncbi Gene Id Number:
4193
Background:
MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 . Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 . Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 .
Background Reference 1:
Haupt, Y. et al. (1997) Nature 387, 296-299. Mayo, L. D. and Donner, D. B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-653

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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