Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of ME1 in 293 cell lysate with ME1 antibody at 1 μg/ml.
Immunohistochemistry of ME1 in mouse liver tissue with ME1 antibody at 5 μg/mL.
Western blot analysis of ME1 in 293 cell lysate with ME1 antibody at 1 μg/ml.
Trial Size

ME1 Antibody

CATALOG NUMBER: 7721

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1.
The immunogen is located within the first 50 amino acids of ME1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 63 kDa
Observed: 63 kDa
Purification:
ME1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ME1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ME1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ME1
Additional Names:
ME1 Antibody: MES, HUMNDME, NADP-dependent malic enzyme, Malic enzyme 1, NADP-ME
Protein Accession Number:
NP_002386
Protein Gi Number:
4505143
Ncbi Gene Id Number:
4199
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1). ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2). It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3). The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4). ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5).
Background Reference 1:
Loeber G, Dworkin MB, Infante A, et al. Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 1994; 344:181-6.
Background Reference 2:
Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
Background Reference 3:
Gonzalez-Manchon C, Butta N, Ferrer M, et al. Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness. DNA Cell Biol. 1997;16:533-44.
Background Reference 4:
Yang Z, Lanks CW and Tong L. Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate. Structure 2002; 10:951-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7721

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369