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Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2μg/ml.
Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 μg/mL.
Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 μg/mL.
Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2μg/ml.
Trial Size

ME2 Antibody

CATALOG NUMBER: 7723

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2.
The immunogen is located within amino acids 340 - 390 of ME2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 64 kDa
Observed: 60 kDa
Purification:
ME2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ME2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ME2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ME2
Additional Names:
ME2 Antibody: ODS1, NAD-dependent malic enzyme, mitochondrial, Malic enzyme 2, NAD-ME
Protein Accession Number:
NP_002387
Protein Gi Number:
4505145
Ncbi Gene Id Number:
4200
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).
Background Reference 1:
Loeber G, Infante AA, Maurer-Fogy I, et al. Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J. Biol. Chem. 1991; 266:3016-21.
Background Reference 2:
Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
Background Reference 3:
Jiang P, Du W, Mancuso A, et al. Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature 2013; 493:689-83.
Background Reference 4:
Lenzen KP, Heils A, Lorenz S, et al. Association analysis of malic acid enzyme 2 gene polymorphisms with idiopathic generalized epilepsy. Epilepsia 2005; 46:1637-41.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7723

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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