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Western blot analysis of MECR in human brain tissue lysate with MECR antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of MECR in (A) human brain tissue, (B) mouse brain tissue, and (C) rat brain tissue lysate with MECR antibody at 1 μg/mL.
Western blot analysis of MECR in human brain tissue lysate with MECR antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

MECR Antibody

CATALOG NUMBER: 7151

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Rabbit polyclonal MECR antibody was raised against a 15 amino acid peptide near the carboxy terminus of human MECR.
The immunogen is located within amino acids 300 - 350 of MECR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MECR antibody can be used for detection of MECR by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of MECR are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 3:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 41 kDa
Purification:
MECR Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MECR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MECR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MECR
Additional Names:
MECR Antibody: NRBF1, CGI-63, FASN2B, NBRF1, Trans-2-enoyl-CoA reductase, mitochondrial, Nuclear receptor-binding factor 1, HsNrbf-1
Protein Accession Number:
NP_057095
Protein Gi Number:
67078404
Ncbi Gene Id Number:
51102
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MECR Antibody: The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.
Background Reference 1:
Masuda N, Yasumo H, Furusawa T, et al. Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors. Gene 1998; 221:225-33.
Background Reference 2:
Chen Z, Leskinen H, Liimatta E, et al. Myocardial overexpression of Mecr, a gene of mitochondrial FAS II leads to cardiac dysfunction in mouse. PLoS One 2009; 4:e5589.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7151

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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