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Western blot analysis in 293 cell line lysates (35ug/lane).
Western blot analysis in 293 cell line lysates (35ug/lane).

MED16 Antibody

CATALOG NUMBER: 58-651

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MED16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human MED16.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
97 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MED16
Additional Names:
Mediator of RNA polymerase II transcription subunit 16, Mediator complex subunit 16, Thyroid hormone receptor-associated protein 5, Thyroid hormone receptor-associated protein complex 95 kDa component, Trap95, Vitamin D3 receptor-interacting protein complex 92 kDa component, DRIP92, MED16, DRIP92 {ECO:0000312, EMBL:AAD310871}, THRAP5
Protein Accession Number:
Q9Y2X0
Protein Gi Number:
62511180
Ncbi Gene Id Number:
10025
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Background Reference 1:
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Background Reference 2:
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Background Reference 3:
Wang, Q., et al. J. Biol. Chem. 277(45):42852-42858(2002)
Background Reference 4:
Kang, Y.K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(5):2642-2647(2002)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-651

List Size:
400 ul

List Price:

$495.00

Datasheet
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