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Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Antibody used in WB on Human Brain at 0.2-1 ug/ml.

MED21 Antibody

CATALOG NUMBER: 28-735

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MED21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MED21 antibody can be used for detection of MED21 by ELISA at 1:62500. MED21 antibody can be used for detection of MED21 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Predicted Molecular Weight:
15 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store MED21 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MED21
Additional Names:
MED21, SRB7, SURB7, hSrb7
Protein Accession Number:
NP_004255
Protein Gi Number:
21361161
Ncbi Gene Id Number:
9412
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MED21 belongs to the Mediator complex subunit 21 family. It is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Background Reference 1:
Andersen, J.S., (2005) Nature 433 (7021), 77-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-735

List Size:
100 ul

List Price:

$519.00

Datasheet
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