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Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 μg/mL.
Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 μg/mL.
Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 μg/mL.
Trial Size

MED4 Antibody

CATALOG NUMBER: 4729

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen:
MED4 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4.
The immunogen is located within the last 50 amino acids of MED4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
MED4 antibody can be used for detection of MED4 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Purification:
MED4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MED4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MED4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MED4
Additional Names:
MED4 Antibody: ARC36, VDRIP, DRIP36, TRAP36, HSPC126, ARC36, Mediator of RNA polymerase II transcription subunit 4, Activator-recruited cofactor 36 kDa component
Protein Accession Number:
NP_054885
Protein Gi Number:
7661788
Ncbi Gene Id Number:
29079
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MED4 Antibody: The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment.
Background Reference 1:
Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell2004; 14:685-91.
Background Reference 2:
Gustafsson CM, Myers LC, Beve J, et al. Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J. Biol. Chem.1998; 273:30851-4.
Background Reference 3:
Rachez C, Lemon BD, Suldan Z, et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature1999; 398:824-8.
Background Reference 4:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4729

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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