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Antibody is used in Western blot to detect MEG2 in A549 cell lysate.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MEG2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Antibody is used in Western blot to detect MEG2 in A549 cell lysate.

MEG2 Antibody

CATALOG NUMBER: 63-456

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MEG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 477-505 amino acids from the C-terminal region of human MEG2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
68 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTPN9
Additional Names:
Tyrosine-protein phosphatase non-receptor type 9, Protein-tyrosine phosphatase MEG2, PTPase MEG2, PTPN9
Protein Accession Number:
P43378
Protein Gi Number:
1172724
Ncbi Gene Id Number:
5780
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MEG2 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal domain that shares a significant similarity with yeast SEC14, which is a protein that has phosphatidylinositol transfer activity and is required for protein secretion through the Golgi complex in yeast. This PTP was found to be activated by polyphosphoinositide, and is thought to be involved in signaling events regulating phagocytosis.
Background Reference 1:
Kruger, J.M., et al., J. Biol. Chem. 277(4):2620-2628 (2002).
Background Reference 2:
Qi, Y., et al., J. Cell. Biochem. 86(1):79-89 (2002).
Background Reference 3:
Gu, M., et al., Proc. Natl. Acad. Sci. U.S.A. 89(7):2980-2984 (1992).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-456

List Size:
400 ul

List Price:

$495.00

Datasheet
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