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Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).
Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).

MEK 2 (phospho Thr394) Antibody

CATALOG NUMBER: 79-106

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MEK-2 (Phospho-Thr394) antibody was raised against a peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
Predicted Molecular Weight:
44 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP2K2
Additional Names:
CFC4, MEK2, MKK2, MAPKK2, PRKMK2, SRC1, ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2
Protein Accession Number:
NP_109587.1
Protein Gi Number:
13489054
Ncbi Gene Id Number:
5605
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Background Reference 1:
Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221. Cowley S, et al. (1994) Cell. 77:841-852.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-106

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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