Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot of NIH 3T3 cell lysates. The cells were serum starved (control) or incubated in the presence of serum (serum) for 5 minutes. The western blot shows the immunolabeling of the ~45k MEK1/2 was absent in control and stimulated by serum.
Western blot of NIH 3T3 cell lysates. The cells were serum starved (control) or incubated in the presence of serum (serum) for 5 minutes. The western blot shows the immunolabeling of the ~45k MEK1/2 was absent in control and stimulated by serum.

MEK1 / 2 (phospho Ser218 / Ser222) Antibody

CATALOG NUMBER: XPS-1018

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Frog, Human, Mouse, Rat
Immunogen:
MEK 1/2 (Ser218/222) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer218/222 of human MEK 1/2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
The antibody immunolabels the ~45k MEK protein in a Western blot of serum-stimulated NIH 3T3 cells. The immunolabeling is blocked by the phosphopeptide used as antigen but not the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
MEK1/2 antibody is specific for MEK 1/2 phosphorylated at Ser218/222.
Predicted Molecular Weight:
45
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
MAP2K1
Protein Accession Number:
Q02750
Protein Gi Number:
400274
Ncbi Gene Id Number:
5604
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MEK 1 and MEK 2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK 1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK 1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK 1/2 is a critical step in the MAP kinase cascade.
Background Reference 1:
N.G. Ahn, N.G., Robbins, D.G., Haycock, J.W., Seger, R., Cobb, M.H. and Krebs, E.G., "Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin," J. Neurochem. 59 (1992) 147 - 156.
Background Reference 2:
Crews, C.M., Alessandrini, A. and Erikson, R.L., "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product," Science 258 (1992) 478 - 480.
Background Reference 3:
Ahn, N.G. "The MAP kinase cascade. Discovery of a new signal transduction pathway," Mol. Cell Biochem. 127 - 128 (1993) 201 - 209.
Background Reference 4:
Adams, J.P. and Sweatt, J.D., "Molecular psychology: Roles for the ERK MAP kinase cascade in memory," Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1018

List Size:
0.1 mL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369