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Western blot analysis of lysed extracts from HepG2 and PC12 cells using MEK1 (Ab-291).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Ab-291).
Western blot analysis of lysed extracts from HepG2 and PC12 cells using MEK1 (Ab-291).

MEK1 (Ab 291) Antibody

CATALOG NUMBER: 79-400

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MEK1 (Ab-291) antibody was raised against a peptide sequence around aa. 289~293 (P-R-T-P-G) derived from Human MEK1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total MEK1 protein.
Predicted Molecular Weight:
45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP2K1
Additional Names:
CFC3, MEK1, MKK1, MAPKK1, PRKMK1, MAPTL, MTBT1, TAU, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1
Protein Accession Number:
NP_002746.1
Protein Gi Number:
5579478
Ncbi Gene Id Number:
5604
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Background Reference 1:
Kevin D. Burroughs, et, al. (2003) Mol. Cancer Res ; 1: 312. Michael J. Piatelli, et, al. (2002) J. Biol. Chem ; 277: 12144 - 12150. Margaret M. Morgan, et, al. (2001) J. Immunol ; 167: 5708. Herbert Schramek, et, al. (2003) Am J Physiol Cell Physiol, ; 285: C652 - C661.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-400

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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